诃子、矮紫堇、甘青乌头提取物对牛病毒性腹泻病毒的体外抑制作用

doi:10.11843/j.issn.0366-6964.2018.09.025

Abstract

Abstract:

This study was conducted to explore anti-BVDV (bovine viral diarrhea virus) effect of three kinds of Tibetan medicine. BVDV was proliferated by virus propagation technology on bovine kidney cells (MDBK) and the TCID₅₀ was detected. The maximum safe concentration and the CC₅₀ value were measured by CCK8 detection and CPE observation. Antiviral inhibition tests were performed by three different modes, adding virus before adding drugs, adding drugs before adding virus, and adding drugs and virus pre-acted for a while in vitro. The Effective inhibition rate, the EC₅₀ value and the therapeutic index (TI) were calculated by statistical analysis software, which would further clarify the antiviral effection of the extracts. The results showed that TCID₅₀ was 10^-4.67·0.1 mL^-1 by the Reed-Muench method. The maximum safe concentrations of Terminalia chebula, Corydalis hendersonii, Aconitum tanguticum, ribavirin were 1 mg·mL^-1, 1 mg·mL^-1, 8 mg·mL^-1 and 2 μg·mL^-1 respectively. All of the maximum inhibition rates of the drugs were more than 50% in virus-and-drug mode. The most effective inhibitory rate treated with Corydalis hendersonii was 72.86% at the dose of 1 mg·mL^-1, which was significantly higher than that of ribavirin (P<0.01). In addition, all of the maximum inhibitory rates were less than 30% in "virus before drugs" and "virus after drugs" mode, indicating unobvious adsorption block effect and unobvious replication blocking effect. In summary, all three kinds of Tibetan medicine had direct killing effect on BVDV. Corydalis hendersonii had the most significant direct inactivated effect and that was superior to ribavirin, which would expect to be further developed into antiviral drugs.

CLC Number:

WANG Dan-yang, ZHANG Kang, WANG Xu-rong, WANG Hai-rui, WANG Lei, ZHANG Kai, ZHANG Jing-yan, LI Jian-xi, WANG Xue-zhi. Inhibitory Effects of Extracts from Terminalia chebula, Corydalis hendersonii, Aconitum tanguticum on Bovine Viral Diarrhea Virus in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 2036-2043.

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Inhibitory Effects of Extracts from Terminalia chebula, Corydalis hendersonii, Aconitum tanguticum on Bovine Viral Diarrhea Virus in vitro

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